Pab pawg tshawb fawb txawv teb chaws luam tawm lub npe" Effect of Valerian Extract on GABRB3 Gene MRNA Expression and Sedation in BALB/C Mice&"; Hauv Tam Sim No Bioactive Compounds thaum Lub Kaum Ib Hlis 10, 2020. Kab lus pom tias GABRB3 noob tom qab ua haujlwm ntawm Valerian extract mRNA qhia tau ntau ntxiv, thiabvalerian extractthiab diazepam muaj cov txiaj ntsig kho mob zoo ib yam.
Valerian yog cov tshuaj ntsuab muaj hnub nyoog tsim tawm hauv Europe thiab Asia. Nws tau faib dav hauv yuav luag txhua lub tebchaws uas cov av muaj acidic. Valerian tau siv hauv cov tshuaj ib txwm muaj los kho kev pw tsaug zog, ntxhov siab, qaug zog, qaug dab peg, qaug dab peg thiab qaug zog rau 2000 xyoo. Lub hauv paus tseem ceeb ntawm cov tshuaj valerian yog valeric acid uas muaj hydroxyl thiab acetone derivatives.
Valeric acid muaj kev tiv thaiv kev ntxhov siab, ua kom tsaug zog thiab ua kom tsaug zog, uas tau lees paub hauv kev tshawb fawb tsiaj thiab kev sim tshuaj. Valeric acid inhibits decomposition γ-aminobutyric acid (GABA) tshwm sim los ntawm catabolic enzymes hauv lub hlwb, yog li ua rau muaj txiaj ntsig sedative. Gamma-aminobutyric acid yog lub hlwb tseem ceeb tshaj plaws cuam tshuam rau kev xa cov neurotransmitter, uas ua lub luag haujlwm tseem ceeb hauv kev tswj hwm thiab ua haujlwm ntawm lub hauv paus paj hlwb. Cov nyhuv sedative ntawm valerian extract tau txhawb los ntawm GABAA receptor β3 subunit. GABRB3 noob belongs rau ligand-gated ion channel tsev neeg thiab nkag mus rau β3 subunit ntawm GABAA receptor. GABRB3 kuj tseem yog tus txais rau diazepam thiab lwm yam tshuaj loog (piv txwv li, phenobarbital).
Valerian cuam tshuam rau cov khoom siv presynaptic ntawm GABAergic neurons, thiab cov khoom no cuam tshuam rau kev tso tawm ntawm synaptic GABA. Valerian tseem txwv qhov rov ua dua thiab catabolism ntawm γ-aminobutyric acid los ntawm kev txwv γ-aminobutyric acid transaminase. Hauv lub hauv paus no, txoj kev tshawb fawb no txhawm rau tshawb fawb cov txiaj ntsig ntawm Valerian rho tawm ntawm kev qhia ntawm GABRB3 noob mRNA thiab kev ua kom loog hauv BALB/c nas.
Hauv qhov kev sim no, tau siv tus qauv tsiaj thiab pab pawg tswj hwm tau teeb tsa los kawm txog cov txiaj ntsig ntawm Valerian rho tawm ntawm kev qhia ntawm GABRB3 noob mRNA thiab sedation hauv BALB/c nas.
Xaiv 20 BALB/c nas thiab faib faib ua 4 pawg; txhua pab pawg muaj 5 nas. Pawg A (kev tswj tsis zoo) tau muab 5 ml dej distilled; pawg B (tswj tau zoo) tau muab 0.025 mg/10 g ntawm diazepam. Pawg C (pab pawg kho mob 1) tau muab valerian extract 2.5 mg/10 g lub cev hnyav, thiab pab pawg D (kho pawg 2) tau muab valerian extract 5 mg/10 g lub cev hnyav. Cov tshuaj no tau muab los ntawm gavage rau 7 hnub. Hnub 7, kev ntsuas lub log tig tau ua tiav. Thawj hnub thiab hnub xya ua ntej kev tswj hwm, cov ntshav ntshav ntawm 1ml tau raug coj los tom qab ntsuas qhov ntsuas, thiab tshuaj xyuas los ntawm RT-PCR.
MRNA qhia txog GABRB3 noob hauv pab pawg B, C, thiab D txhua qhov nce ntxiv (p< 0.0001),="" thiab="" muaj="" qhov="" sib="" txawv="" tseem="" ceeb="" ntawm="" pab="" pawg="" c="" thiab="" d.="" sib="" txawv="" ntawm="" pab="" pawg="" a="" thiab="" pab="" pawg="" b,="" pab="" pawg="" a="" thiab="" pab="" pawg="" c,="" pab="" pawg="" a="" thiab="" pab="" pawg="" d="">< 0.05).="" tsis="" muaj="" qhov="" sib="" txawv="" tseem="" ceeb="" ntawm="" pab="" pawg="" b="" thiab="" pab="" pawg="" c="" thiab="">
Kev qhia ntawm GABRB3 noob mRNA tau nce ntau ntxiv tom qab kev nqis tes ua ntawm Valerian extract. Raws li kev ntsuas lub log tig, valerian extract thiab diazepam muaj cov tshuaj kho mob zoo ib yam. Kev siv tshuaj valerian ntau dua yuav tsis tsim qib siab dua ntawm GABRB3 noob mRNA qhia, thiab nws yuav tsis tsim cov nyhuv sedative.
